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siRNA Synthesis
As a leading provider in the field of oligonucleotide therapeutics, Protheragen BioNucleics offers comprehensive small interfering RNA (siRNA) synthesis services to accelerate your research endeavors. Our extensive experience in Oligonucleotide Drugs CRO and Oligonucleotide Sequence Synthesis Service enables us to deliver high-quality, custom siRNA molecules tailored to your specific needs.
Overview of siRNA Synthesis
siRNA molecules are double-stranded RNA sequences, typically 20–25 nucleotides in length, that play a pivotal role in gene silencing through the RNA interference (RNAi) pathway. By directing the degradation of complementary mRNA sequences, siRNA allows researchers to selectively knock down gene expression, making it a powerful tool for functional genomics studies and therapeutic applications. Precision in synthesis and modification is crucial to ensure the efficacy and specificity of siRNA molecules.
Our Services
Custom siRNA Design and Consultation
We provide expert consultation to design siRNA sequences with optimal gene-silencing efficiency and minimal off-target effects. Our bioinformatics team utilizes advanced algorithms to predict siRNA efficacy, ensuring that the designed sequences achieve maximum potency against your target gene.
High-Fidelity siRNA Synthesis
Leveraging state-of-the-art solid-phase synthesis technology, we produce siRNA with high accuracy and consistency. Our synthesis process includes:
- Strand Synthesis: Both sense and antisense strands are synthesized with precise nucleotide sequences.
- Chemical Modifications: We offer a variety of modifications to enhance siRNA stability and functionality, such as 2'-O-methyl, 2'-fluoro, locked nucleic acids (LNAs), and phosphorothioate backbones.
- Labeling Options: Fluorescent labels (e.g., FAM, Cy5) and other tags can be incorporated for tracking and imaging purposes.
Annealing and Quality Control
Post-synthesis, the single-stranded RNAs are carefully annealed to form the functional siRNA duplex. Rigorous quality control measures are implemented to confirm:
- Purity: Using high-resolution analytical techniques to ensure the absence of synthesis impurities.
- Integrity: Verification of correct sequence and structure through mass spectrometry and gel electrophoresis.
- Concentration Accuracy: Precise quantification of siRNA concentration using spectrophotometric analysis.
Scale and Packaging Options
We accommodate various project scales, from small-scale research quantities to large-scale production suitable for preclinical studies. Our packaging options are designed for convenience and longevity, including:
- Lyophilized Powder: For extended storage and stability.
- Solution Formulations: siRNA delivered in your choice of buffer.
Additional Support Services
In addition to the above services, we also provide many additional support services, including but not limited to:
- siRNA Library Construction: Custom synthesis of siRNA libraries targeting multiple genes or entire gene families.
- miRNA Inhibitors and Mimics: Synthesis of specialized RNA molecules for microRNA research.
- Delivery System Development: Assistance with designing and optimizing siRNA delivery methods, such as nanoparticle encapsulation.
Frequently Asked Questions
Q1: How do you ensure the quality and purity of synthesized siRNA?
Quality assurance is embedded at every stage. We employ advanced chromatographic and spectroscopic methods to verify siRNA purity, integrity, and sequence accuracy. Our protocols adhere to global regulatory guidelines, ensuring removal of contaminants such as truncated sequences or residual solvents.
Q2: Can siRNA be customized with chemical modifications for specific therapeutic needs?
Absolutely. Our platform supports a wide array of chemical modifications to enhance siRNA stability, delivery, and target engagement. This includes sugar and backbone modifications to resist degradation, as well as tissue-specific conjugates for precise biodistribution. Modifications are strategically designed to maintain RNA interference activity while minimizing off-target interactions, ensuring a balance between efficacy and safety.
Applications of siRNA
Oncology
Silencing oncogenes or resistance markers in solid tumors.
Antiviral Therapies
Targeting conserved viral RNA regions (e.g., HBV, SARS-CoV-2)
Rare Genetic Disorders
Correcting splicing defects in diseases like amyloidosis.
Neurological Diseases
Reducing toxic protein aggregates in Alzheimer's or ALS.
Metabolic Disorders
Modulating liver-specific genes in hypercholesterolemia.
Why Choose Us?
- End-to-End Solutions: From design to delivery, all under one roof.
- AI-Driven Design Tools: Minimize off-target risks computationally.
- Collaborative Partnerships: Co-development models to accelerate your pipeline.
Our services and products are exclusively for authorized organizations in research, development, or manufacturing and are not intended for direct use by individuals or patients or as medical advice, diagnosis, or treatment.
