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Please note that we are not a pharmacy or clinic, so we are unable to see patients and do not offer diagnostic and treatment services for individuals.

siRNA 3' End Modification

At Protheragen BioNucleics, we specialize in providing comprehensive solutions for oligonucleotide-based therapeutics. Our expertise spans across Oligonucleotide Drugs CRO, Oligonucleotide Modification Service, siRNA Modification Service, and specifically siRNA End Modification. We are committed to delivering high-quality, customized services to accelerate your research and development in the field of RNA interference (RNAi).

Overview of siRNA 3' End Modification

Small interfering RNAs (siRNAs) are powerful tools for gene silencing, but their stability and delivery efficiency can be challenging. Modifying the 3' end of siRNA molecules enhances their nuclease resistance, improves pharmacokinetic properties, and enhances cellular uptake. These modifications can include the addition of chemical groups, conjugation with molecules like cholesterol, or incorporation of modified nucleotides, all aimed at optimizing the siRNA's therapeutic potential.

Our Comprehensive siRNA 3' End Modification Services

Custom 3' End Chemical Modifications

We offer a wide range of chemical modifications at the 3' end of siRNA, including:

3' End Phosphorylation: Enhances stability against exonucleases.
Attachment of Functional Groups: Such as amino, thiol, or azide groups for further conjugation.
Incorporation of Modified Nucleotides: Including 2'-O-methyl, locked nucleic acids (LNAs), or phosphorothioates to increase binding affinity and stability.

Synthesis of siRNA with Diverse Modifications

We specialize in the synthesis of siRNAs incorporating multiple types of 3' end modifications to meet complex research needs:

Dual Modifications: Combining chemical modifications and conjugations for synergistic effects.
Fluorescent Labeling: For tracking and imaging studies.
Biotinylation: Enables purification or immobilization on streptavidin-coated surfaces.

Quality Control and Analytical Services

Ensuring the highest quality of modified siRNA is our priority:

Mass Spectrometry Analysis: Confirms molecular weight and modification integrity.
HPLC Purification: Guarantees high purity of the final product.
Functional Validation: Assessing gene silencing efficiency in relevant cellular models.

Customized Solutions and Technical Support

Our team of experts works closely with clients to develop tailored solutions:

Consultation Services: Advising on the optimal modification strategies for specific applications.
Rapid Turnaround Times: Efficient project management to meet tight deadlines.
Dedicated Support: Ongoing assistance throughout your research project.

Frequently Asked Questions

Q1: How do 3' end modifications improve siRNA stability and efficacy?

Modifying the siRNA 3' end protects it from exonucleases, enzymes that degrade RNA molecules. Chemical modifications and protective groups enhance stability, allowing siRNA to persist longer in biological systems. This increases the opportunity for effective gene silencing by maintaining active concentrations of siRNA.

Q2: Will 3' end modifications interfere with the siRNA's ability to silence target genes?

When designed correctly, 3' end modifications do not hinder siRNA function. Our experts ensure that modifications enhance stability without affecting the RNA-induced silencing complex (RISC) loading or target recognition, preserving or even improving silencing efficiency.

Q3: Can you assist with the design of siRNA modifications specific to my project?

Yes, we offer collaborative support to tailor siRNA modifications to your project's requirements. Our team provides consultation on modification strategies that align with your goals, whether for increased stability, targeted delivery, or minimal immunogenicity.

Applications of siRNA 3' End Modification

Gene Function Studies

Increased efficiency in gene knockdown experiments.

Development of RNAi-Based Drugs

Optimizing candidates for preclinical development.

Biological Research Tools

Fluorescently labeled siRNA for imaging studies.

Diagnostic Applications

Modified siRNA probes for detecting gene expression.

Molecular Imaging

Using labeled siRNA for non-invasive imaging techniques to track distribution and efficacy in real time.

Our services and products are exclusively for authorized organizations in research, development, or manufacturing and are not intended for direct use by individuals or patients or as medical advice, diagnosis, or treatment.