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ASO Synthesis
At Protheragen BioNucleics, we specialize in providing comprehensive solutions for oligonucleotide-based therapeutic development. Our expertise in Oligonucleotide Drugs CRO and Oligonucleotide Sequence Synthesis Service positions us at the forefront of antisense oligonucleotide (ASO) synthesis. We are committed to delivering high-quality, customized ASOs to accelerate your research.
Overview of ASO Synthesis
Antisense oligonucleotides are short, synthetic strands of nucleic acids designed to bind specifically to target RNA molecules, modulating gene expression and offering therapeutic potential for a variety of diseases. ASO synthesis involves intricate chemical processes to produce oligonucleotides with high purity, stability, and specificity. Key aspects include:
- Sequence Design: Crafting oligonucleotides that complement target RNA sequences.
- Chemical Modifications: Enhancing stability and reducing degradation through backbone and sugar modifications.
- Purification and Quality Control: Employing advanced techniques to ensure the integrity and efficacy of the ASOs.
Our Comprehensive ASO Synthesis Services
At Protheragen BioNucleics, we offer end-to-end ASO synthesis services tailored to your specific needs.
Custom ASO Design and Optimization
- Target Identification: Utilizing advanced bioinformatics tools, we identify optimal target sites on the mRNA of interest.
- Sequence Optimization: Designing sequences to enhance binding affinity and specificity while minimizing off-target effects.
- Chemical Modification Selection: Incorporating modifications such as 2'-O-methyl, 2'-O-methoxyethyl (2'-MOE), locked nucleic acids (LNAs), and phosphorothioate backbones to improve nuclease resistance and pharmacokinetic properties.
Automated Solid-Phase Oligonucleotide Synthesis
- Advanced Synthesizers: Employing the latest synthesizers for precise and efficient ASO production.
- High Throughput Capability: Capable of synthesizing large libraries of ASOs with varying lengths and modifications.
- Quality Monitors: Integrating real-time monitoring systems to ensure consistency and accuracy throughout the synthesis process.
Advanced Purification Techniques
- High-Performance Liquid Chromatography (HPLC): Utilizing reverse-phase and ion-exchange HPLC for high-purity ASO isolation.
- Gel Electrophoresis: Implementing polyacrylamide gel electrophoresis (PAGE) for size-based purification.
- Desalting Procedures: Removing impurities and salts to enhance ASO stability and performance.
Comprehensive Analytical Characterization
- Mass Spectrometry: Conducting MALDI-TOF and ESI-MS analyses for molecular weight confirmation.
- Nuclear Magnetic Resonance (NMR): Assessing structural integrity and conformation.
- UV Spectrophotometry: Determining concentration and purity through absorbance measurements.
Stability Studies and Formulation Development
- Stability Testing: Performing accelerated and long-term stability studies under different conditions.
- Formulation Optimization: Developing suitable delivery systems to enhance bioavailability and target specificity.
Technical Support and Consultation
- Expert Guidance: Offering consultation on ASO design strategies and modification selection.
- Troubleshooting Assistance: Providing support to address any challenges encountered during research and development.
Frequently Asked Questions
Q1: What chemical modifications can improve ASO stability and efficacy?
Incorporating modifications such as phosphorothioate backbones, 2'-O-methyl, 2'-MOE, and LNAs can enhance nuclease resistance, binding affinity, and overall stability of ASOs.
Q2: How do you ensure the specificity of the synthesized ASOs?
We utilize advanced sequence alignment tools during design to minimize homology with non-target sequences and perform rigorous analytical validation post-synthesis to confirm specificity.
Applications
- Gene Silencing: Targeting pathogenic mRNA to inhibit the expression of disease-causing genes.
- Therapeutic Development: Formulating treatments for genetic disorders, including Duchenne muscular dystrophy.
- Cancer Therapy: Disrupting oncogene expression in various cancers for more effective treatment outcomes.
- Viral Infections: Designing ASOs to target viral RNA in diseases like hepatitis and HIV.
- Cardiovascular Disorders: Investigating ASOs for the modulation of lipid metabolism and cholesterol levels.
Our services and products are exclusively for authorized organizations in research, development, or manufacturing and are not intended for direct use by individuals or patients or as medical advice, diagnosis, or treatment.
