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Please note that we are not a pharmacy or clinic, so we are unable to see patients and do not offer diagnostic and treatment services for individuals.

miRNA Sugar Modification

At Protheragen BioNucleics, we specialize in providing comprehensive solutions in the field of oligonucleotide therapeutics. As a leading Oligonucleotide Drugs CRO, we offer a wide range of services, including Oligonucleotide Modification Service and specialized miRNA Modification Service. Our expertise in miRNA sugar modification enables us to support your research and development projects with cutting-edge technologies and unparalleled expertise.

Overview of MicroRNAs (miRNAs) Sugar Modification

miRNAs are small, non-coding RNA molecules that play crucial roles in regulating gene expression. Modifying the sugar moiety of miRNAs enhances their stability, bioavailability, and affinity for target mRNAs, making them more effective for therapeutic applications. Sugar modifications such as 2'-O-methylation, 2'-fluoro modifications, and locked nucleic acid (LNA) incorporation improve resistance to nucleases, reduce immunogenicity, and increase cellular uptake. Understanding these modifications is essential for developing effective miRNA-based therapeutics that can overcome the challenges of delivery and stability in biological systems.

Our Services

Custom miRNA Synthesis with Sugar Modifications

We offer custom synthesis of miRNAs incorporating various sugar modifications to enhance their therapeutic potential. Our portfolio includes:

2'-O-Methylation: Enhances nuclease resistance and reduces immunogenicity, leading to improved stability in biological fluids.
2'-Fluoro Modification: Increases binding affinity to target mRNAs and enhances resistance to enzymatic degradation.
2'-O-Methoxyethyl (2'-MOE) Modification: Offers superior stability and potency for in vivo applications.
Locked Nucleic Acid (LNA) Incorporation: Significantly increases thermal stability and binding affinity, making them highly effective in gene regulation studies.

Design and Optimization Support

Our team of experienced scientists provides expert guidance on the design and optimization of miRNA sequences with appropriate sugar modifications. We work closely with you to achieve the desired biological activity while minimizing off-target effects and immunogenic responses.

Comprehensive Analytical Services

We ensure the highest quality of synthesized miRNAs through rigorous quality control processes, including:

Mass Spectrometry Analysis: Confirms the molecular weight and structural integrity of modified miRNAs.
High-Performance Liquid Chromatography (HPLC): Ensures purity and consistency of the synthesized products.
Functional Assays: Validates the biological activity and effectiveness of the modified miRNAs in relevant cellular models.

Frequently Asked Questions

Q1: What are the advantages of using locked nucleic acid (LNA) modifications in miRNA studies?

LNA modifications significantly enhance the thermal stability and binding affinity of miRNAs to their target mRNAs. This results in increased specificity and potency in gene regulation applications. LNAs also improve resistance to nucleases, leading to greater stability in biological systems, which is crucial for both research and therapeutic purposes.

Q2: How do sugar modifications affect the immunogenicity of miRNA therapeutics?

Certain sugar modifications, such as 2'-O-methylation, can reduce the immunogenicity of miRNA molecules. By altering the sugar backbone, these modifications prevent recognition by pattern recognition receptors of the immune system, thereby minimizing unwanted immune responses and enhancing the safety profile of miRNA therapeutics.

Q3: Can you assist with selecting the most appropriate sugar modification for my specific application?

Yes, our expert team provides personalized consultation to help you select the most suitable sugar modification based on your research goals, target cells or tissues, and desired biological outcomes. We consider factors such as stability requirements, target affinity, and delivery mechanisms to ensure optimal results.

Applications

Therapeutic Development

Enhancing miRNA stability and efficacy for novel drug development in conditions like cancer, cardiovascular diseases, and neurological disorders.

Gene Expression Studies

Investigating gene regulation mechanisms by using stable and effective miRNA molecules.

Biomarker Discovery

Utilizing modified miRNAs for the detection and quantification of disease-associated biomarkers.

Functional Genomics

Validating gene function and interactions through targeted miRNA modulation.

Why Choose Us

Expertise and Experience: With a team of seasoned professionals, we bring extensive knowledge in miRNA biology and sugar modification techniques.
Customized Solutions: We tailor our services to meet the unique requirements of your project, ensuring optimal outcomes.
Rigorous Quality Control: We adhere to stringent quality standards to deliver reliable and reproducible results.

Our services and products are exclusively for authorized organizations in research, development, or manufacturing and are not intended for direct use by individuals or patients or as medical advice, diagnosis, or treatment.