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miRNA Synthesis
At Protheragen BioNucleics, we are dedicated to advancing genetic research and therapeutic development through our expertise in oligonucleotide synthesis. Our miRNA synthesis services play a pivotal role in supporting innovative research and drug development pipelines. To learn more about our comprehensive offerings, please visit our Oligonucleotide Drugs CRO and Oligonucleotide Sequence Synthesis Service pages.
Overview of miRNA Synthesis
MicroRNAs (miRNAs) are small, non-coding RNA molecules typically 20-24 nucleotides in length. They regulate gene expression post-transcriptionally by binding to complementary sequences on target messenger RNAs (mRNAs), leading to mRNA degradation or translational repression. This regulation is crucial in various biological processes, including development, cell differentiation, proliferation, and apoptosis.
Synthesizing miRNAs is essential for researchers studying gene function and for developing miRNA-based therapeutics. Synthetic miRNAs allow for the manipulation of gene expression in vitro and in vivo, facilitating the exploration of cellular pathways and disease mechanisms. At Protheragen BioNucleics, we provide high-quality, customized miRNA synthesis services that meet the specific needs of your research and development projects.
Our Services
Our services are dedicated to providing comprehensive miRNA synthesis solutions, including custom miRNA synthesis, modified miRNA molecules, design and synthesis of miRNA mimics and inhibitors, high-throughput miRNA library synthesis, and strict quality control and documentation support. Utilizing advanced synthesis platforms and a professional technical team, we ensure the delivery of miRNA products with high fidelity and purity.
Custom miRNA Synthesis
We offer fully customized miRNA synthesis tailored to your specific sequences and requirements. Our advanced synthesis platforms ensure high fidelity and purity, providing miRNAs that are ready for immediate use in your experiments. Services include:
- Sequence Design Assistance: Collaborate with our experts to design optimal miRNA sequences for your targets.
- Flexible Synthesis Scales: Choose from various scales ranging from nanomole to micromole quantities to suit your project needs.
- Modifications and Labeling: Enhance miRNA stability and detection with chemical modifications and fluorescent or biotin labels.
Modified miRNA Molecules
Chemical modifications can significantly improve miRNA performance by enhancing stability, binding affinity, and resistance to nuclease degradation. Our offerings include:
- 2'-O-Methyl and 2'-Fluoro Modifications: Increase nuclease resistance and target binding specificity.
- Phosphorothioate Linkages: Enhance stability by replacing non-bridging oxygen atoms with sulfur in the backbone.
- Locked Nucleic Acids (LNAs): Incorporate LNAs to improve thermal stability and hybridization properties.
miRNA Mimics and Inhibitors
Manipulate endogenous miRNA activity using our synthetic miRNA mimics and inhibitors:
- miRNA Mimics: Double-stranded molecules that simulate endogenous miRNAs, upregulating gene silencing pathways.
- miRNA Inhibitors: Single-stranded, chemically modified oligonucleotides that bind to and inhibit specific miRNAs.
High-Throughput miRNA Library Synthesis
Accelerate large-scale screening projects with our high-throughput synthesis capabilities:
- miRNA Libraries: Custom or pre-designed libraries targeting multiple miRNAs for comprehensive analysis.
- Rapid Turnaround: Efficient synthesis processes ensure timely delivery for time-sensitive projects.
Quality Control and Documentation
Ensure confidence in your results with our rigorous quality assurance protocols:
- Analytical Validation: Each miRNA is analyzed using mass spectrometry and capillary electrophoresis to confirm identity and purity.
- Documentation: Receive detailed reports including synthesis data, analytical results, and handling instructions.
Frequently Asked Questions
Q1: What information is required to place a custom miRNA synthesis order?
You need to provide the desired miRNA sequence, any specific modifications or labels, purity requirements, and quantity needed.
Q2: Can you synthesize miRNA mimics and inhibitors?
Yes, we specialize in synthesizing both miRNA mimics for gene upregulation studies and inhibitors for gene silencing applications.
Q3: How do you ensure the quality of synthesized miRNAs?
We employ stringent quality control measures, including mass spectrometry and HPLC, to verify the purity and integrity of every miRNA product.
Our services and products are exclusively for authorized organizations in research, development, or manufacturing and are not intended for direct use by individuals or patients or as medical advice, diagnosis, or treatment.
