Services

Online Inquiry

Your Name

Phone Number

* E-mail Address

* Services Interested

Project Description

Please note that we are not a pharmacy or clinic, so we are unable to see patients and do not offer diagnostic and treatment services for individuals.

siRNA 5' End Modification

At Protheragen BioNucleics, we specialize in providing comprehensive solutions for oligonucleotide-based therapeutics. Our expertise in Oligonucleotide Drugs CRO, Oligonucleotide Modification Services, and siRNA Modification Services positions us at the forefront of innovative drug development. Specifically, our advanced capabilities in siRNA End Modification empower researchers to enhance the stability and efficacy of siRNA molecules for therapeutic applications.

Overview of siRNA 5' End Modification

The 5' end modification of small interfering RNA (siRNA) is a critical technique in the enhancement of siRNA-based therapeutics. By modifying the 5' end of siRNA strands, we can significantly improve their stability against nucleases, enhance cellular uptake, and reduce off-target effects. These modifications play a vital role in tailoring siRNA molecules for specific therapeutic needs, ensuring efficient gene silencing with minimal side effects. Our focus on 5' end modification enables the development of siRNA therapeutics with superior pharmacokinetic properties and increased therapeutic potential.

Our Services

We offer a comprehensive suite of siRNA 5' end modification services designed to meet the diverse needs of our clients in research and drug development. Our state-of-the-art technologies and experienced team provide high-quality, customized siRNA molecules with precise 5' end modifications.

5' Phosphorylation and Dephosphorylation

5' Phosphorylation: Enhances the incorporation of siRNA into the RNA-induced silencing complex (RISC) by adding a phosphate group to the 5' end, improving gene silencing efficiency.
5' Dephosphorylation: Involves the removal of the phosphate group to study its impact on siRNA activity and stability.

5' Chemical Modifications

5' End Capping with Inverted Nucleotides: Protects siRNA from exonuclease degradation by incorporating inverted nucleotides at the 5' end, increasing molecule stability without affecting function.
5' Fluorophore Labeling: Attaches fluorescent dyes to the 5' end for visualization and tracking of siRNA delivery and localization within cells.
5' Biotinylation: Adds a biotin moiety for affinity purification and detection applications, facilitating the study of siRNA interactions.

Incorporation of Modified Nucleotides

2'-O-Methyl and 2'-Fluoro Modifications: Incorporates chemically modified nucleotides at the 5' end to enhance nuclease resistance and reduce immunogenicity, improving therapeutic efficacy.
Locked Nucleic Acids (LNAs): These are used to increase binding affinity to target mRNA, enhancing gene silencing potency.

Custom Design and Synthesis

Sequence Optimization: Provides assistance in designing siRNA sequences with optimal silencing efficiency and minimal off-target effects.
Dual Modifications: Offers the combination of multiple modifications at the 5' end to address complex therapeutic challenges.

Quality Control and Assurance

We adhere to stringent quality control measures to ensure the highest standards of our siRNA products.

Purity Assessment: Utilizes high-performance liquid chromatography (HPLC) and mass spectrometry to confirm the purity and correct molecular weight of modified siRNA.
Functional Validation: Performs in vitro assays to verify gene silencing efficiency and specificity of the modified siRNA.
Endotoxin Testing: Ensures that all products are free from endotoxin contamination, making them suitable for sensitive biological applications.

Technical Support

Our dedicated team of scientists provides comprehensive support throughout your project.

Consultation Services: Offers expert guidance on selecting appropriate 5' end modifications based on your specific research goals.
Troubleshooting Assistance: Provides solutions to any challenges encountered during experimentation with our modified siRNA products.
Data Analysis Support: Aids in the interpretation of experimental results to maximize the value of your research.

Frequently Asked Questions

Q1: How do 5' end modifications enhance siRNA stability?

5' end modifications protect siRNA molecules from degradation by exonucleases, enzymes that degrade nucleic acids from their ends. By adding chemical groups or inverted nucleotides at the 5' end, we block exonuclease activity, thereby increasing the siRNA's stability in biological environments and prolonging its gene-silencing effect.

Q2: Do you offer custom modifications beyond the listed services?

Absolutely. We provide custom siRNA modification services tailored to your specific research needs. Whether you require uncommon chemical groups or dual modifications, our team is equipped to design and synthesize siRNA molecules that meet your exact specifications.

Our services and products are exclusively for authorized organizations in research, development, or manufacturing and are not intended for direct use by individuals or patients or as medical advice, diagnosis, or treatment.