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Please note that we are not a pharmacy or clinic, so we are unable to see patients and do not offer diagnostic and treatment services for individuals.

Oligonucleotide Sequence Synthesis Service

Oligonucleotides are short sequences of nucleotides that are fundamental tools in molecular biology, genetics, and biotechnology. They serve as primers in polymerase chain reactions (PCR), probes for detecting specific DNA or RNA sequences, and as therapeutic agents in antisense therapy and gene silencing. As part of our Oligonucleotide Drugs CRO Services, Protheragen BioNucleics provides high-quality and customized oligonucleotide synthesis services to support cutting-edge research and development in these fields.

The Importance of Oligonucleotide Synthesis

Advancements in genomics and molecular diagnostics have amplified the demand for precise and reliable oligonucleotide synthesis. High-quality oligonucleotides are crucial for:

PCR and qPCR Assays: Facilitating the amplification and quantification of specific DNA sequences.
Next-Generation Sequencing (NGS): Enabling library preparation and target enrichment.
Gene Editing: Providing guide RNAs for gene editing systems.
Antisense Oligonucleotide Therapies: Modulating gene expression for therapeutic purposes.
Microarrays and DNA Chips: Analyzing gene expression profiles on a large scale.

Our Synthesis Platform

At Protheragen BioNucleics, we utilize advanced synthesis technologies to deliver oligonucleotides with exceptional quality:

Automated Solid-Phase Synthesis: Ensuring high efficiency and accuracy in oligonucleotide assembly.
High-Purity Reagents: Utilizing premium-grade phosphoramidites and coupling agents for optimal synthesis performance.
Scalable Production: Offering synthesis scales from micrograms to grams to meet various project needs.

Customization and Modification Services

Understanding that each research project has unique requirements, we provide extensive customization options. We provide Oligonucleotide Monomer Synthesis, siRNA Synthesis, miRNA Synthesis, ASO Synthesis, and Aptamer Synthesis.

Sequence Length and Composition: Synthesizing oligonucleotides ranging from 5 to 200 bases with any desired sequence.
Chemical Modifications: Including 5' and 3' end modifications, backbone modifications, and internal labels:
- Fluorescent Labels: Such as FAM, HEX, TAMRA for detection and imaging applications.
- Quenchers and Dark Dyes: For constructing molecular beacons and probes.
- Locked Nucleic Acids (LNA): Enhancing binding affinity and specificity.
Conjugation Services: Attaching peptides, biotin, or other molecules to expand functionality.

Synthesis Technology

Oligonucleotide sequences can be synthesized using liquid-phase or solid-phase techniques.

Liquid-phase synthesis assembles oligonucleotide sequences entirely in solution without using a solid support, offering unique advantages. It provides greater flexibility for incorporating diverse chemical modifications—such as unusual bases, linkers, and labels—due to adjustable reaction conditions. By reducing steric hindrance present in solid-phase methods, it enables the synthesis of longer oligonucleotides with higher coupling efficiencies. Enhanced control over reaction intermediates facilitates purification and analysis throughout the process.
Solid-phase synthesis involves attaching the first nucleotide to an insoluble resin support, enabling sequential addition of nucleotides with intermediates and excess reagents easily removed by washing. Solid-phase synthesis is efficiency, automation potential, and suitability for producing high-purity oligonucleotides.

Rigorous Quality Control Measures

Quality is at the forefront of our services at Protheragen BioNucleics. We implement stringent quality control protocols to ensure the highest standards:

Analytical HPLC and Mass Spectrometry: Confirming purity and molecular weight of synthesized oligonucleotides.
UV Spectrophotometry: Accurately determining oligonucleotide concentration.
Certificate of Analysis (CoA): Providing detailed documentation for every product delivered.

Applications Supported by Our Services

Our high-quality oligonucleotides empower a wide range of applications:

Diagnostic Development: Crafting probes and primers for detecting genetic diseases and pathogens.
Therapeutic Research: Developing antisense oligonucleotides, siRNA, and aptamers for novel treatments.
Agricultural Biotechnology: Enhancing crop traits through gene editing and molecular breeding.
Academic and Research: Facilitating fundamental studies in gene function and regulation.

Our Advantages

Expert Consultation: Offering guidance on oligonucleotide design and application strategies.
Fast Turnaround Times: Ensuring timely delivery to keep your projects on schedule.
Customized Solutions: The commitment to offering customized solutions plays a significant role in addressing the unique challenges faced by researchers.

Protheragen BioNucleics stands out as a trusted provider of oligonucleotide sequence synthesis services, tailored to meet the complex needs of modern biotechnology and pharmaceutical research. Contact us today to explore how our expertise can accelerate your scientific endeavors.

Our services and products are exclusively for authorized organizations in research, development, or manufacturing and are not intended for direct use by individuals or patients or as medical advice, diagnosis, or treatment.