Services

Online Inquiry

Your Name

Phone Number

* E-mail Address

* Services Interested

Project Description

Please note that we are not a pharmacy or clinic, so we are unable to see patients and do not offer diagnostic and treatment services for individuals.

siRNA Phosphodiester Bond Modification

At Protheragen BioNucleics, we are dedicated to pushing the boundaries of oligonucleotide therapeutics. Our expertise in Oligonucleotide Drugs CRO services, Oligonucleotide Modification Services, and siRNA Modification Services positions us at the forefront of innovative solutions in siRNA phosphodiester bond modification.

Overview of siRNA Phosphodiester Bond Modification

Small interfering RNA (siRNA) has emerged as a powerful tool for gene silencing, offering immense potential in therapeutic applications. However, the inherent instability and susceptibility to nuclease degradation pose significant challenges for clinical use. Phosphodiester bond modification involves altering the backbone of siRNA molecules to enhance their stability, bioavailability, and specificity. By modifying the phosphate backbone, we can improve resistance to nucleases, reduce off-target effects, and optimize the pharmacokinetic properties essential for effective siRNA therapeutics.

Our Services

At Protheragen BioNucleics, we offer comprehensive services in siRNA phosphodiester bond modification, employing state-of-the-art technologies to deliver high-quality, customized solutions for your research and therapeutic needs.

Phosphorothioate Modification

We provide phosphorothioate backbone modifications, where one of the non-bridging oxygen atoms in the phosphate group is replaced with sulfur. This modification enhances nuclease resistance and improves the pharmacokinetic profile of siRNA molecules. Phosphorothioate-modified siRNA exhibits increased stability in biological fluids, making it a valuable tool for in vivo applications.

2'-O-Methyl Phosphoramidate Modifications

Our team specializes in introducing 2'-O-methyl phosphoramidate linkages, which enhance siRNA stability and decrease immunogenicity without compromising target specificity. This modification reduces off-target effects and improves siRNA's interaction with the RNA-induced silencing complex (RISC), enhancing gene silencing efficiency.

Borane Phosphonate Modification

We offer borane phosphonate backbone modifications to further increase nuclease resistance and improve the therapeutic potential of siRNA molecules. This unique modification provides enhanced stability while maintaining the ability to activate RISC, essential for effective gene silencing.

Advanced Synthesis Technologies

Utilizing cutting-edge solid-phase and liquid-phase synthesis techniques, we ensure high purity and accuracy in modified siRNA production. Our advanced purification processes, including high-performance liquid chromatography (HPLC) and mass spectrometry verification, guarantee that the modified siRNA meets stringent quality standards required for research applications.

Custom Design and Optimization

Our experts work closely with you to design and optimize siRNA sequences with tailored phosphodiester bond modifications. We consider factors such as target gene, delivery method, and desired therapeutic outcome to provide personalized solutions. Our goal is to maximize efficacy while minimizing potential side effects.

Frequently Asked Questions

Q1: What are the benefits of phosphodiester bond modification in siRNA?

Phosphodiester bond modification enhances siRNA stability by increasing resistance to nuclease degradation, improves pharmacokinetics, reduces immunogenicity, and maintains or enhances target specificity.

Q2: How does phosphorothioate modification differ from other backbone modifications?

Phosphorothioate modification replaces non-bridging oxygen with sulfur, enhancing nuclease resistance and cellular uptake. It differs from other modifications like borane phosphonate or phosphoramidate linkages in its chemical structure, which can influence the siRNA's interaction with cellular components and its overall efficacy.

Q3: Do you offer assistance with siRNA design and optimization?

Absolutely. Our team of experts assists clients in designing and optimizing siRNA sequences with appropriate phosphodiester bond modifications. We tailor our services to your specific needs, ensuring optimal performance for your intended application.

Q4: How do you ensure the quality of the modified siRNA?

We implement rigorous quality control measures, including purity assessment, sequence verification, and stability testing. Our advanced analytical techniques guarantee that our modified siRNA meets the highest standards of quality and performance.

Our services and products are exclusively for authorized organizations in research, development, or manufacturing and are not intended for direct use by individuals or patients or as medical advice, diagnosis, or treatment.