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Please note that we are not a pharmacy or clinic, so we are unable to see patients and do not offer diagnostic and treatment services for individuals.

Aptamer Synthesis

At Protheragen BioNucleics, we specialize in providing comprehensive solutions for oligonucleotide-based therapeutic development. Our expertise extends to advanced aptamer synthesis, a critical tool in drug development and diagnostic applications. Explore our Oligonucleotide Drugs CRO and Oligonucleotide Sequence Synthesis Service for more information on how we support your research needs.

Understanding Aptamer Synthesis

Aptamers are short, single-stranded DNA or RNA molecules that bind to specific targets with high affinity and specificity. synthesized through an iterative process known as systematic evolution of ligands by exponential enrichment (SELEX), aptamers offer several advantages over traditional antibodies, including smaller size, lower immunogenicity, and ease of chemical modification. These properties make them invaluable in various biomedical applications.

Our Comprehensive Aptamer Synthesis Service

As an ISO9001 certified supplier, Protheragen BioNucleics offers a full spectrum of aptamer synthesis services tailored to your project's requirements.

Custom Aptamer Design and Synthesis

We provide bespoke aptamer sequences designed using advanced bioinformatics tools. Our team ensures optimal binding characteristics by target analysis, sequence optimization, and chemical modifications.

SELEX Library Construction

Our expertise in SELEX library construction enables the generation of high-diversity nucleic acid libraries:

Randomized Libraries: Creating libraries with randomized regions up to 100 nucleotides.
Diverse Pool Generation: Ensuring a broad representation of sequences to increase the likelihood of identifying high-affinity aptamers.
Quality Control: Utilizing next-generation sequencing (NGS) to assess library diversity.

In Vitro SELEX Technology

We employ state-of-the-art in vitro SELEX techniques to isolate aptamers against a wide range of targets:

Iterative Selection: Conducting multiple rounds of binding, partitioning, and amplification.
Affinity Enhancement: Refining conditions to progressively enrich high-affinity binders.
Counter-Selection Strategies: Eliminating non-specific binders to enhance specificity.

Aptamer Characterization and Optimization

Our services include comprehensive characterization to validate aptamer functionality:

Binding Affinity Assessment: Using surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC).
Structural Analysis: Employing circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopy.
Optimization Services: Modifying sequences for enhanced stability and reduced immunogenicity.

Scale-Up Synthesis and Purification

We offer scalable synthesis solutions to meet your production needs:

Custom Synthesis Scales: Providing quantities from milligrams to grams.
High-Purity Purification: Utilizing high-performance liquid chromatography (HPLC) and capillary electrophoresis.
Quality Assurance: Performing rigorous analytical testing to ensure product integrity.

Aptamer Conjugation and Labeling

Enhance the functionality of your aptamers with our conjugation services:

Fluorescent Labeling: For use in imaging and diagnostic assays.
Biotinylation: Facilitating immobilization and capture applications.
Nanoparticle Conjugation: Enabling targeted drug delivery systems.

Frequently Asked Questions

Q1. What information is required to initiate an aptamer synthesis project?

We need details about your target molecule, including its structure and desired binding characteristics. Any specific modifications or applications for the aptamer should also be communicated.

Q2. Can you modify existing aptamers to improve their performance?

Yes, we offer optimization services for existing aptamers, including sequence modification and chemical alterations to enhance stability and affinity.

Q3. How do you ensure the quality and purity of synthesized aptamers?

Our synthesis and purification processes include stringent quality control measures, such as HPLC and mass spectrometry, to guarantee high purity and conformity to specifications.

Applications of Aptamers

Therapeutic Agents

Targeting specific proteins or cells in disease treatment.

Diagnostic Tools

Serving as biosensors for detecting biomarkers.

Drug Delivery Systems

Facilitating targeted delivery of therapeutics.

Research Reagents

Acting as molecular probes in biochemical assays.

Our services and products are exclusively for authorized organizations in research, development, or manufacturing and are not intended for direct use by individuals or patients or as medical advice, diagnosis, or treatment.