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Project Description

Please note that we are not a pharmacy or clinic, so we are unable to see patients and do not offer diagnostic and treatment services for individuals.

Oligonucleotide Modification Service

At Protheragen BioNucleics, we specialize in providing cutting-edge oligonucleotide modification services to accelerate the development of next-generation therapeutics. As a leading Oligonucleotide Drugs CRO, we are committed to delivering high-quality, customized solutions that meet the diverse needs of our clients in the pharmaceutical and biotechnology sectors

Overview of Oligonucleotide Modification Services

Oligonucleotides have revolutionized the field of molecular medicine, offering precise tools for gene regulation and therapeutic intervention. However, native oligonucleotides often face challenges such as poor stability, low bioavailability, and off-target effects. Our oligonucleotide modification services address these issues by enhancing the physicochemical properties of oligonucleotides, thereby improving their therapeutic potential. By incorporating various chemical modifications, we enhance stability against nucleases, increase binding affinity, and optimize pharmacokinetics, ensuring more effective and reliable results in research.

Our Services

We offer a comprehensive suite of oligonucleotide modification services tailored to the specific needs of different therapeutic modalities:

siRNA Modification Service

Small interfering RNA (siRNA) is a powerful tool for gene silencing. Our siRNA modification services enhance the stability and efficacy of siRNA molecules. We provide modifications such as 2'-O-methylation, phosphorothioate backbones, and lipid conjugation to improve resistance to nucleases, reduce immunogenicity, and enhance cellular uptake.

miRNA Modification Service

MicroRNAs (miRNAs) play crucial roles in gene regulation. Our miRNA modification services aim to improve the stability and functional performance of miRNAs in therapeutic applications. Modifications include locked nucleic acids (LNAs), 2'-fluoro, and 2'-O-methoxyethyl groups, which increase binding affinity and specificity towards target mRNAs.

ASO Modification Service

Antisense oligonucleotides (ASOs) are designed to modulate gene expression. Our ASO modification services focus on enhancing target recognition and stability. We offer chemical modifications like gapmers, peptide nucleic acids (PNAs), and morpholino phosphorodiamidate linkages to improve hybridization properties and resistance to enzymatic degradation.

ASO Sugar Modification
ASO Nucleotide Base Modification
ASO Backbone Modification

Aptamer Modification Service

Aptamers are oligonucleotide molecules that bind specific targets with high affinity. Our aptamer modification services enhance their stability and binding characteristics. By introducing modifications such as pegylation, sulfhydryl, and biotin labels, we improve pharmacokinetics and facilitate conjugation to other molecules or surfaces for therapeutic and diagnostic applications.

Aptamer Sugar Modification
Aptamer Nucleotide Base Modification
Aptamer End Modification

Our Process

Our streamlined and collaborative approach ensures that clients receive high-quality modified oligonucleotides tailored to their specific needs:

Consultation and Design

The process begins with a detailed consultation to understand the project's objectives and requirements. Our team of experts collaborates closely with clients to design oligonucleotides with the most suitable modifications that align with their research goals.

Customization and Synthesis

Based on the agreed design, we proceed with the synthesis of the modified oligonucleotides. Utilizing advanced synthesis platforms and chemistries, we incorporate the desired modifications with high precision and efficiency.

Quality Control and Validation

Every synthesized oligonucleotide undergoes rigorous quality control procedures. We employ analytical techniques such as high-performance liquid chromatography (HPLC), mass spectrometry, and capillary electrophoresis to verify purity, sequence accuracy, and successful incorporation of modifications.

Delivery and Support

Upon completion of quality validation, the final product is delivered promptly to the client. We provide comprehensive documentation and are available for ongoing support to address any questions or further needs that may arise during the application of the modified oligonucleotides.

Frequently Asked Questions

Q1: What types of chemical modifications can be incorporated into oligonucleotides?

We offer a wide range of chemical modifications, including backbone modifications (phosphorothioate, morpholino), sugar modifications (2'-O-methyl, 2'-fluoro, LNA), base modifications (5-methylcytosine, 5-propynyl uridine), and conjugations (fluorescent dyes, biotin, lipids). Our team can work with you to determine the most appropriate modifications for your specific application.

Q2: How do modified oligonucleotides improve therapeutic efficacy?

Chemical modifications enhance the stability of oligonucleotides against nuclease degradation, increase binding affinity to target nucleic acids, reduce immunogenicity, and improve cellular uptake. These improvements lead to increased bioavailability and potency, ultimately enhancing therapeutic efficacy.

Our services and products are exclusively for authorized organizations in research, development, or manufacturing and are not intended for direct use by individuals or patients or as medical advice, diagnosis, or treatment.